Transfer of bacteria from one container to another. This room is meant for inoculation of bacteria i.e. The electron microscope is installed in this room and the room is air-conditioned. Great care is taken in this room, so that the pure cultures do not get contaminated with other bacteria, which, otherwise, would lead to erroneous results. Isolated pure cultures of unknown bacteria (stock cultures) are also maintained in this room for further identification. The standard pure cultures of known bacteria obtained from the international laboratories are maintained in the stock culture room. It is given a new name (a new genera, or new species or new strain) based on its closeness to the known bacteria. If a pure culture of unknown bacteria is found to differ from similar standard pure cultures of known bacteria in morphology, staining reactions, biochemical reactions and serological tests, then it is sent to the international standard laboratories.Īfter thorough testing in these laboratories, if it is found to differ from the known bacteria, then it is declared ‘new’ and is published in the International Journal of Systematic Bacteriology of the American Society of Microbiologists. These laboratories maintain and supply the standard pure cultures of known bacteria. In routine microbiological analysis, very often it is required to isolate different types of bacteria in samples, to maintain the isolated bacteria as pure cultures and to identify them in subsequent days by performing several tests.Īfter identification, it is required to compare them with standard pure cultures of the same species obtained from international standard microbiology laboratories such as, American Type Culture Collection (ATCC) of the USA National Collection of Type Cultures (NCTC) of England and Pasteur Culture Collection (PCC) of France. Other equipments, such as single-pan precision balance, Quebec colony counter, electronic colony counter, particle counter, electrophoresis apparatus, UV-cum-visible double beam spectrophotometer, computer, gas chromatography (GC), high performance liquid chromatography (HPLC), pH meter, trinocular research microscope with photomicrography attachment, projection microscope, fluorescence microscope, dark-field microscope, phase-contrast microscope and PCR thermo cycler should be arranged on a continuous concrete shelf projecting from the wall as shown in Figure 3.1. The instruments to be kept on the floor of the room include fridge, refrigerated centrifuge and ultracentrifuge. Otherwise, accumulation of dust particles on and inside the instruments as well as exposure to high room temperatures decreases the longevity of the instruments.Īt the same time, high humid conditions lead to the rusting of their metallic components and fungal growth on optical parts, such as microscope lenses and phototubes of spectrophotometers. It should be air-conditioned to make it dust-free and to reduce humidity as well as to avoid high ambient temperatures. This room should be kept neat and clean, as most of the sophisticated instruments are kept in it. should be kept on the shelf as shown in Figure 3.1. Space should be left out on the concrete shelf for different purposes labeled accordingly such as, ‘waste to be sterilized’, ‘sterilized materials’, ‘materials to be sterilized’, ‘light microscopy’, ‘glassware cleaning’ etc.Įquipments, such as oven, microbiological incubator, BOD incubator, single- pan balance, double-pane electrical balance, ultrapure water purification system, distilled water plant, shaking water bath, TLC apparatus, UV lamp, magnetic stirrer homogenizer etc. At one corner of the lab, an autoclave, preferably a double-jacketed horizontal autoclave should be kept. A dispose jar containing germicide solution such as lysol with a thick layer of cotton at its bottom should be kept on or near each table for the pipettes to be disposed into it after use.
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